Walter F. Stafford, III, Ph.D

Senior Scientist & Director of Computer Science
Breast cancer, Huntington’s Disease, basic cellular functions

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Research Summary
My main interests are in the application of physical methods to study protein-protein and protein-nucleic acid interactions that are relevant to physiological function in normal and diseased states. Highly sensitive analytical techniques for the analytical ultracentrifuge have been developed by me at BBRI and are being applied to these problems. Ongoing development of mathematical methods, software algorithms and hardware, including optics and electronics, is continuing to improve the usefulness of these techniques.

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Selected Publications
Stafford, W.F. (2009) Protein-protein and protein-ligand interactions studied by analytical ultracentrifugation in Protein Structure, Stability, and Interactions, J. W. Shriver, editor, Vol. 490, Methods in Molecular Biology series, pp 83-113, Humana (Springer), New York.

Houmeida A, Baron A, Keen J, Khan GN, Knight PJ, Stafford WF 3rd, Thirumurugan K, Thompson B, Tskhovrebova L, Trinick J. (2008) Evidence for the oligomeric state of ‘elastic’ titin in muscle sarcomeres. J Mol Biol. 384(2):299-312.

Adams GP, Tai MS, McCartney JE, Marks JD, Stafford WF 3rd, Houston LL, Huston JS, Weiner LM. (2006) Avidity-mediated enhancement of in vivo tumor targeting by single-chain Fv dimers. Clin Cancer Res. 12(5):1599-605.

Liu H, Stafford WF, Bouvier M. (2005) The endoplasmic reticulum lumenal domain of the adenovirus type 2 E3-19K protein binds to peptide-filled and peptide-deficient HLA-A*1101 molecules. J Virol. 79(21):13317-25.

Knight PJ, Thirumurugan K, Xu Y, Wang F, Kalverda AP, Stafford WF 3rd, Sellers JR, Peckham M. (2005) The predicted coiled-coil domain of myosin 10 forms a novel elongated domain that lengthens the head. J Biol Chem. 280(41):34702-8.

Mukhopadhyay S, Langsetmo K, Stafford WF 3rd, Henry GD, Baleja JD, Sarkar S. (2005) Identification of a region of fast skeletal troponin T required for stabilization of the coiled-coil formation with troponin I. J Biol Chem. 280(1):538-47.

Stafford WF, Walker ML, Trinick JA, Coluccio LM. (2005) Mammalian class I myosin, Myo1b, is monomeric and cross-links actin filaments as determined by hydrodynamic studies and electron microscopy. Biophys J. 88(1):384-91.

Gelinas AD, Toth J, Bethoney KA, Stafford WF, Harrison CJ. (2004) Mutational analysis of the energetics of the GrpE.DnaK binding interface: equilibrium association constants by sedimentation velocity analytical ultracentrifugation. J Mol Biol. 339(2):447-58.