James L. Sherley, M.D., Ph.D.
Senior Scientist
Email: sherleyj at bbri. org

Research Summary
Our laboratory pursues normal molecular and biochemical processes in adult stem cells that are involved in cancer initiation and that contribute to aging. Adult stem cells are rare tissue cells that continuously replace expired tissue cells. Investigations of their specialized properties will yield new therapies for injured, diseased, and aging tissue cells. We employ an integrated approach, incorporating both basic and applied research strategies, to elucidate novel mechanisms of adult stem cell-specific functions and apply the knowledge to improve methods for identifying adult stem cells and producing them in large number for therapeutic development.  

Direct visualization of non-random immortal DNA co-segregation
in an experimental model for adult stem cell functions.

Selected Publications
Sherley, J. L. (2006). Mechanisms of genetic fidelity in mammalian adult stem cells. In Tissue Stem Cells (Potten, C. S., Clarke, R. B., Wilson, J. & Renehan, A. G., eds.), pp. 37-54. Taylor Francis, New York.

Pare, J. F. & Sherley, J. L. (2006). Biological principles for ex vivo adult stem cell expansion. Curr Top Dev Biol 73, 141-171.

Rambhatla, L., Ram-Mohan, S., Cheng, J. J. & Sherley, J. L. (2005). Immortal DNA strand cosegregation requires p53/IMPDH-dependent asymmetric self-renewal associated with adult stem cells. Cancer Res 65, 3155-3161.

Lee, H. S., Crane, G. G., Merok, J. R., Tunstead, J. R., Hatch, N. L., Panchalingam, K., Powers, M. J., Griffith, L. G. & Sherley, J. L. (2003). Clonal expansion of adult rat hepatic stem cell lines by suppression of asymmetric cell kinetics (SACK). Biotechnol Bioeng 83, 760-771.

Merok, J. R., Lansita, J. A., Tunstead, J. R. & Sherley, J. L. (2002). Cosegregation of chromosomes containing immortal DNA strands in cells that cycle with asymmetric stem cell kinetics. Cancer Res 62, 6791-3795.

PubMed:
Click here for a list of publications (searches the National Library of Medicine's PubMed database.)