Molecular motors, hearing
Our laboratory studies myosin-I, a class of molecular motors implicated in cell motility, establishment of polarity, and actin organization. Some years ago we isolated three class I myosins now known as Myo1b, Myo1c, and Myo1d from rat liver. Since then we have used a combination of biochemical, molecular and cellular biological approaches to understand the mechanism and function of these enzymes. Our studies have shown that these molecules, which are associated with several subcellular fractions, are kinetically very slow and well-suited for maintenance of tension in the cytoskeleton. Recently, we showed how the biophysical properties of Myo1c, which has a specialized function in the inner ear, enable it to support adaptation, a requirement for vestibular function.
Adamek, N., L. M. Coluccio and M. A. Geeves. 2008. Calcium-sensitive ATP hydrolysis of Myo1c, the adaptation motor in the inner ear. Proc. Natl. Acad. Sci. U S A 105:5710-5715. View Full Text (PDF).
Coluccio, L. M. 2008. Myosin I. In Myosins: A superfamily of molecular motors, L. M. Coluccio, editor, Vol. 7, Proteins and cell regulation series, pp 95-124, Springer Verlag, The Netherlands.
Coluccio, L. M. 2008. Myosins: A superfamily of molecular motors, Editor. Vol. 7, Proteins and cell regulation series, Springer Verlag, The Netherlands. For info about this book.
Lieto-Trivedi, A., S. Dash and L. M. Coluccio. 2007. Myosin surface loop 4 modulates inhibition of acto-myosin 1B ATPase activity by tropomyosin. Biochemistry 46:2779-2786.
Coluccio, L. M. 2006. Myo1b. AfCS-Nature Molecule Pages. (doi:10.1038/mp.a001573.01).
Clark, R., M., S. Ansari, S. Dash, M. A. Geeves, and L. M. Coluccio. 2005. Loop 1 of transducer region in Myo1b modulates actin affinity, ATPase activity, and nucleotide access. J. Biol. Chem. 280:30935-30942.
Stafford, W. S., M. Walker, J. Trinick and L. M. Coluccio. 2005. Mammalian class I myosin, Myo1b, is monomeric and crosslinks actin filaments as determined by hydrodynamic studies and electron microscopy. Biophys. J. 88:384-391.
Batters, C., M. I. Wallace, L. M. Coluccio, and J. E. Molloy. 2004b. A model of stereocilia adaptation based on single molecule mechanical studies of myosin-I. Phil. Trans. R. Soc. B. 359:1895-1905.
Batters, C., C. P. Arthur, A. Lin, J. Porter, M. A. Geeves, R. A. Milligan, J. E. Molloy, and L. M. Coluccio. 2004a. Myo1c is tuned for the adaptation response in the inner ear. EMBO J. 23:1433-1440.