Transformation for CaCl2 Cells

  1. Pipet 50ul CaCl2 competent cells and 1ul of DNA into a cold tube.
  2. Place on ice for 20 minutes.
  3. Heat @ 37C for 3 minutes.
  4. Add 250ul of LB.
  5. Shake for one hour
  6. Plate on Carban. and Chloranphenicol plates